Fig. 3

Combination of GST-SlWRKY28 fusion protein with W-box element. In the gel block, the lanes were followed by GST + protein and biotin-labeled oligonucleotide probe (Biotin-Pr); GST-SlWRKY28 fusion protein and Biotin-Pr were added; also undiluted oligonucleotide probe (Cold-Pr) diluted 10-fold; added GST-SlWRKY28 fusion protein and Biotin-Pr, and 50-fold diluted unlabeled oligonucleotide probe (Cold-Pr). The thin arrow refers to the DNA and protein complex; the thick arrow refers to the free probe